Hepatitis C Nucleic Acid Amplification Testing (NAAT) for Acute Case Detection: A Historical Protocol Repurposed to Combat a Current Outbreak

Hepatitis C Nucleic Acid Amplification Testing (NAAT) for Acute Case Detection: A Historical Protocol Repurposed to Combat a Current Outbreak

LB-5. Hepatitis C Nucleic Acid Amplification Testing (NAAT) for Acute Case Detection: A Historical Protocol Repurposed to Combat a Current Outbreak Jessica Gentry, MA; Sara Blosser, PhD; Kathleen Backfish, BS, (M)CM; Michael Cross, BS; Marta Cruz, MT(ASCP); Edwin Hartle II,; Erica Vecchio, MS; Erika Chapman, MPH, CPH, CHES; Caitlin Conrad, BS; Joan Duwve, MD, MPH; Romeo Galang, MD, MPH; Daniel ...

nucleic acid testing for the detection of hbv dna

Real-time nucleic acid sequence-based amplification assay for detection of hepatitis A virus.

A nucleic acid sequence-based amplification (NASBA) assay in combination with a molecular beacon was developed for the real-time detection and quantification of hepatitis A virus (HAV). A 202-bp, highly conserved 5' noncoding region of HAV was targeted. The sensitivity of the real-time NASBA assay was tested with 10-fold dilutions of viral RNA, and a detection limit of 1 PFU was obtained. The s...

nucleic acid-based approaches for detection of viral hepatitis

conclusions despite rapidity, automation, accuracy, cost-effectiveness, high sensitivity, and high specificity of molecular techniques, each type of molecular technology has its own advantages and disadvantages. results various types of molecular biology tools can be used to detect and quantify viral genomic elements and analyze the sequences. these molecular assays are proper technologies for ...

Integrated self-regulating resistive heating for isothermal nucleic acid amplification tests (NAAT) in Lab-on-a-Chip (LoC) devices

Isothermal nucleic acid amplification tests (NAAT) in a Lab-on-a-Chip (LoC) format promise to bring high-accuracy, non-instrumented rapid tests to the point of care. Reliable rapid tests for infectious diseases allow for early diagnosis and treatment, which in turn enables better containment of potential outbreaks and fewer complications. A critical component to LoC NAATs is the heating element...